Score 750 points or more on a single kill (Multiplayer Only).
Kill your target and escape your pursuer in less than 10 seconds (Multiplayer Only).
Take the lead 10 seconds before the end of a session and go on to win (Multiplayer Only).
Kill your target while hidden in a hay bale (Multiplayer Only).
Kill a guard with the bag that drops from a lift.
Kill 10 guards with a single arrow storm.
Solve all of Subject 16’s puzzles.
Throw a Long Weapon, Heavy Weapon, and Smoke bomb more than 10 m at a guard.
Beat up the 5 thirsty harlequins.
Discover the Shrine in the Cloaca Maxima.
Train a recruit up to the Rank of Assassin.
Discover the Shrine in Palazzo Laterano.
Discover the Shrine in Il Colosseo.
Discover the Shrine in the Catacombe di Roma.
Discover the Shrine in the Basilica di San Pietro.
Jump with the parachute off the top of Castel Sant'Angelo.
Kill a guard with a broom.
Destroy the Bomber.
Destroy the Tank.
Throw money in a well.
Complete an entire Session with at least one kill (Multiplayer Only).
Destroy the Naval Cannon.
Secure the Apple of Eden.
Complete DNA Sequence 9.
Complete DNA Sequence 8.
Complete DNA Sequence 7.
Burn All Borgia Towers.
Complete DNA Sequence 6.
Complete DNA Sequence 5.
Win 10000 florins playing Hazard.
Complete DNA Sequence 4.
Destroy the Machine Gun.
Recruit 3 Assassins.
Renovate 5 buildings in the Antico district.
Find 1 Artifact in 2012.
Access your email in 2012.
Complete DNA Sequence 3.
Win the highest bet at the Fights.
Complete DNA Sequence 2.
Achieve 100% Synchronization in any Sequence other than Sequence 1.
Discover the Shrine in the Terme di Traiano.
Replay a Memory.
Find a secure place to hide and re-enter the Animus.
Complete DNA Sequence 1.
Attempt to access DNA Sequence 9 for the first time.
Win every trophy.
Complete 10 Guild Challenges.
Earn 3 gold medals in the Animus Virtual Training Program.
Remove All Borgia Flags in Rome.
Collect All Feathers.
Perform an Execution Streak of 10 kills.
During Open Conflict, kill your target and escape (Multiplayer Only).
Reach Level 50 (Multiplayer Only).
Get all the Co-op bonuses in 1 session (Multiplayer Only).
Get every single bonus at least once (Multiplayer Only).
Perform a double or triple escape (Multiplayer Only).
Receive 100% synch in ACB and full synch in all missions of the Da Vinci Disappearance.
Complete the Da Vinci Disappearance.
Double Assassinate from a parachute.
Steal 5 horses from their riders, while remaining on horseback.
Pay 500 florins to an orator, then pickpocket him afterward.